Development of immunoassay based biosensors for sensitive detection of EDCs (Endocrine Disrupting Chemicals) Mecoprop and Bisphenol A.
The aim of the project TOXsense is to develop a prototype of a mobile toxsensor using a microfluidic device as a platform for detection and quantification of Bisphenol A (BPA), firstly in water samples.
The design of the microfluidic device enables in contrast to conventional methods the measurement of large volumes of fluid samples and is simultaneously timesaving. The toxsensor is based on a competitive immunoassay, with specific antibodies covalently immobilized inside a reaction chamber which accumulate BPA specifically.
Microfluidic devices were fabricated by using a photo resin, which contains epoxy groups that ensure a covalent protein binding to the surface.
The detection limit of the immunoassay is 0.5ng/ml and the signal is induced by an enzyme tracer reacting with a colorimetric substrate.
The great advantage of the developed microfluidic device is its easy adaption to other toxins or pathogens simply by changing the specific antibodies. The final toxsensor is supposed to be an on-site device for fieldwork, fast, reliable and user-friendly. Especially for monitoring of aquatic environments, it can be a useful tool for supervisory authorities, industrial companies or research facilities.
This project is funded by the state of Bremen, Germany by the support program “applied environmental research” and the European funds for regional development EFRE 2007-2013.